Aldehyde dehydrogenases (ALDHs) comprise a superfamily of 19 enzymes that catalyze the irreversible oxidation of various aldehydes to their corresponding carboxylic acids. This class includes the retinaldehyde dehydrogenases (RALDHs). The three class one (cytosolic) ALDHs that synthesize retinoic acid (ALDH1A1, ALDH1A2, and ALDH1A3; a.k.a., respectively, RALDH1, RALDH2, and RALDH3) are involved in growth and development of many major organ systems. There have been no specific inhibitors for the class one ALDH enzymes. Disulfiram, citral, and DEAB have been used to inhibit retinoic acid synthesis in experimental settings, but they demonstrate poor isoform selectivity resulting in numerous off target effects.